Protein Blotting and Detection - Paperback

EAN (ISBN-13): 9781617379437
ISBN (ISBN-10): 1617379433
Hardcover
Paperback
Publishing year: 2010
Publisher: Humana Press Inc.

Book in our database since 2014-02-22T07:13:23-05:00 (New York)
Detail page last modified on 2023-09-14T04:21:48-04:00 (New York)
ISBN/EAN: 9781617379437

ISBN - alternate spelling:
1-61737-943-3, 978-1-61737-943-7
Alternate spelling and related search-keywords:
Book author: scofield

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Information from Publisher

Author: Biji T. Kurien
Title: Methods in Molecular Biology; Protein Blotting and Detection - Methods and Protocols
Publisher: Humana; Humana Press
588 Pages
Publishing year: 2011-01-26
Totowa; NJ; US
Printed / Made in
Language: English
181,49 € (DE)

BC; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Immunoblotting; Membrane proteomics; Polyacrylamid-Gelelektrophorese; Protein transfer; Proteomics; SDS-PAGE; TLC-Blot; Western blotting; molecular biology; Protein Biochemistry; BB; EA

Origins of Protein Blotting.- Western Blotting: Remembrance of Things Past.- to Protein Blotting.- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis.- Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support.- Tryptic Peptide Purification Using Polyvinylidene Difluoride Membrane for Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry.- Affinity Immunoblotting for Analysis of Antibody Clonotype Distribution in a Lupus Patient Developing Anti-Ro 60 Over Time.- Nonelectrophoretic Bidirectional Transfer of a Single SDS-PAGE Gel with Multiple Antigens to Obtain 12 Immunoblots.- Slice Blotting.- Localizing Proteins by Tissue Printing.- Dot-Immunobinding Assay.- Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes After Agarose-Gel Electrophoresis Separation.- Quantitative computerized western blotting.- Cationic electrophoresis and eastern blotting.- A miniaturized blotting system for simultaneous detecting of differentautoantibodies.- Transfer and Multiplex Immunoblotting of a Paraffin Embedded Tissue.- Multistrip Western Blotting to Increase Quantitative Data Output.- Western Blotting/Edman Sequencing using PVDF Membrane.- Blotting from PhastGel to Membranes by Ultrasound.- Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat.- Strip Immunoblotting of Multiple Antigenic Peptides to Nitrocellulose Membrane.- Affinity Purification of Autoantibodies from an Antigen Strip Excised from a Nitrocellulose Protein Blot.- Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures.- Efficient Electroblottingof Very Large Proteins Using a Vertical Agarose Electrophoresis System.- Semi-Dry Protein Transfer and Immunodetection of P-Selectin Using an Antibody to its C-terminal Tag.- The Multiple Antigen Blot Assay: A Simple, Versatile and Multipurpose Immunoenzymatic Technique.- Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates.- Immunoprecipitation-Western Blot for Proteins of Low Abundance.- Native Electrophoresis and Western Blot Analysis: Method and Applications.- Grid-Immunoblotting.- Sequential Use of Immunoblots for Characterization of Autoantibody Specificities.- Renaturation of Recombinant Ro 60 Autoantigen by Calcium Ions on PVDF Membrane.- Intermittent Microwave Irradiation Facilitates Antigen-Antibody Reaction in Western Blot Analysis.- Detection of Protein-Protein Interactions by Far-Western Blotting.- On-Membrane Tryptic Digestion of Proteins for Mass Spectrometry Analysis.- Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay.- ELISPOT Assay on Membrane Microplates.- A Brief Review of Other Notable Protein Blotting Methods.- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore.- Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation.- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting.- Whole and Strip Nitrocellulose Membrane as well as a New-Line-Immunoblotting ofAntigen Using the Chemiluminescence Technique.- Protein Stains to Detect Antigen on Membranes.- Use of non-radioactive detection method for North- and South-Western Blot.- Immunoblotting using Radiolabeled Reagents for Detection.- Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods.- “Rainbow” Western Blotting.- Multiple antigen detection (MAD) western blotting.- Detection of calcium binding by Ro 60 multiple antigenic peptides on nitrocellulose membrane using Quin-2-.- Detection of La/SS-B by Western Blot using Nanogold-Tagged Antibodies andSilver Enhancement.- Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection.- A Fluorescent Codetection System for Immunoblotting and Proteomics Through ECL-Plex and CyDye Labeling.- Visualization of Unstained Protein Bands on PVDF.- Improved Protein Detection Using Cold Microwave Technology.- TLC Blot (Far-Eastern Blot) and Its Applications.- A Brief Review of Other Notable Protein Detection Methods on Blots.- Sending Secret Messages on Nitrocellulose Membrane and the Use of a Molecular Pen for Orientation in ECL Membrane Assays.