Two-Dimensional Electrophoresis Protocols - Paperback

EAN (ISBN-13): 9781617378492
ISBN (ISBN-10): 1617378496
Hardcover
Paperback
Publishing year: 2010
Publisher: Humana Press Inc.

Book in our database since 2014-06-26T16:26:11-04:00 (New York)
Detail page last modified on 2021-11-25T08:47:34-05:00 (New York)
ISBN/EAN: 9781617378492

ISBN - alternate spelling:
1-61737-849-6, 978-1-61737-849-2
Alternate spelling and related search-keywords:
Book author: sheehan
Book title: electrophoresis

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Information from Publisher

Author: David Sheehan; Raymond Tyther
Title: Methods in Molecular Biology; Two-Dimensional Electrophoresis Protocols
Publisher: Humana; Humana Press
546 Pages
Publishing year: 2010-11-19
Totowa; NJ; US
Printed / Made in
Language: English
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XIV, 546 p. 100 illus., 1 illus. in color.

BC; Hardcover, Softcover / Biologie/Biochemie, Biophysik; Biochemie; Verstehen; Chromosome; Polyacrylamid-Gelelektrophorese; Proteomics; Translation; algorithms; bioinformatics; biological materials; databases; evolution; molecular biology; post-translational modification; sequence analysis; Electrophoresis; Biochemistry; Protein Biochemistry; Separation Science; Analytische Chemie; BB

Two-Dimensional Electrophoresis: An Overview.- Solubilization of Proteins in 2DE: An Outline.- Selection of pH Ranges in 2DE.- Difficult Proteins.- Organelle Proteomics.- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry.- Immunoblotting 2DE Membranes.- Troubleshooting Image Analysis in 2DE.- Analysis of Bacterial Proteins by 2DE.- Proteomic Analysis of Caenorhabditis elegans.- Protein Extraction for 2DE.- Analysis of Proteins from Marine Molluscs.- Preparation and Analysis of Plant and Plastid Proteomes by 2DE..- High-Resolution 2DE.- Blue Native-Gel Electrophoresis Proteomics.- 2DE for Proteome Analysis of Human Metaphase Chromosomes.- Microsomal Proteomics.- Prefractionation Using Microscale Solution IEF.- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges.- High-Resolution Large-Gel 2DE.- Silver Staining of Proteins in 2DE Gels.- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach.- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells.- Detection of Ubiquitination in 2DE.- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry.- Detection of Protein Glutathionylation.- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases.- Active Protease Mapping in 2DE Gels.- Two-Dimensional Difference Gel Electrophoresis.- Protein Expression Profiling.- C-Terminal Sequence Analysis of 2DE-Separated Proteins.- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry.- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination.- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis.- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations.- Creating 2DE Databases for the World Wide Web.
Covers a wide biological range of proteome sources including microbes, plants and animals Addresses key aspects of experimental design Includes some of the latest approaches such as DIGE and detection of post-translational modification Includes supplementary material: sn.pub/extras