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CRISPR - Herausgegeben:Fineran, Peter Charpentier, Emmanuelle Lundgren, Magnus
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Herausgegeben:Fineran, Peter Charpentier, Emmanuelle Lundgren, Magnus:

CRISPR - hardcover

2015, ISBN: 9781493926862

[ED: Hardcover], [PU: Humana / Springer New York / Springer, Berlin], This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applicati… More...

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CRISPR - Magnus Lundgren
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Magnus Lundgren:

CRISPR - new book

2020, ISBN: 9781493926862

[ED: Buch], [PU: Springer New York], Neuware - This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, gen… More...

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CRISPR Methods and Protocols - Lundgren, Magnus (Herausgeber); Fineran, Peter C. (Herausgeber); Charpentier, Emmanuelle (Herausgeber)
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Lundgren, Magnus (Herausgeber); Fineran, Peter C. (Herausgeber); Charpentier, Emmanuelle (Herausgeber):
CRISPR Methods and Protocols - hardcover

2015

ISBN: 1493926861

2015 Gebundene Ausgabe Erblehre, Genetik, Genetik / Humangenetik, Humangenetik, CRISPR-Casdefensesystems; CRISPR-Cas9technology; CRISPR-basedgenetictools; DNA/protein; RNA/protein; arch… More...

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CRISPR Methods and Protocols - Lundgren, Magnus (Herausgeber); Fineran, Peter C. (Herausgeber); Charpentier, Emmanuelle (Herausgeber)
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Lundgren, Magnus (Herausgeber); Fineran, Peter C. (Herausgeber); Charpentier, Emmanuelle (Herausgeber):
CRISPR Methods and Protocols - hardcover

2015, ISBN: 1493926861

2015 Gebundene Ausgabe Erblehre, Genetik, Genetik / Humangenetik, Humangenetik, protein/proteininteractions; geneexpression; CRISPR-Casdefensesystems; CRISPR-Cas9technology; CRISPR-base… More...

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CRISPR - Magnus Lundgren; Emmanuelle Charpentier; Peter C. Fineran
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Magnus Lundgren; Emmanuelle Charpentier; Peter C. Fineran:
CRISPR - hardcover

2015, ISBN: 9781493926862

Methods and Protocols, Buch, Hardcover, 2015 ed. [PU: Humana Press Inc.], Humana Press Inc., 2015

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Details of the book
CRISPR
Author:
Title:
ISBN:

9781493926862


This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels. CRISPR: Methods and Protocols guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from standard protocols of DNA, RNA and protein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, CRISPR: Methods and Protocols provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

Details of the book - CRISPR


EAN (ISBN-13): 9781493926862
ISBN (ISBN-10): 1493926861
Hardcover
Paperback
Publishing year: 2015
Publisher: Humana Press Inc.

Book in our database since 2015-10-06T16:23:49-04:00 (New York)
Detail page last modified on 2024-02-24T10:00:53-05:00 (New York)
ISBN/EAN: 9781493926862

ISBN - alternate spelling:
1-4939-2686-1, 978-1-4939-2686-2
Alternate spelling and related search-keywords:
Book author: lund, lun, fine, charpentier, lundgren, peter von magnus, emmanuelle

Information from Publisher

Author: Magnus Lundgren; Emmanuelle Charpentier; Peter C. Fineran
Title: Methods in Molecular Biology; CRISPR - Methods and Protocols
Publisher: Humana; Springer US
366 Pages
Publishing year: 2015-05-20
New York; NY; US
Printed / Made in
Language: English
192,59 € (DE)
197,99 € (AT)
212,50 CHF (CH)
POD
XIV, 366 p. 63 illus., 37 illus. in color.

BB; Hardcover, Softcover / Biologie/Genetik, Gentechnik; Genetik (nicht-medizinisch); Verstehen; CRISPR-Cas defense systems; CRISPR-Cas9 technology; CRISPR-based genetic tools; DNA/protein; RNA/protein; archaeal species; bacterial species; genetic elements; horizontal gene transfer; protein/protein interactions; gene expression; Molecular Genetics; EA; BC

Investigating CRISPR RNA Biogenesis and Function using RNA-seq.- In vitro Co-reconstitution of Cas Protein Complexes.- Analysis of CRISPR pre-crRNA Cleavage.- Annotation and Classification of CRISPR-Cas Systems.- Computational Detection of CRISPR/crRNA Targets.- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium.- Spacer-based Macroarrays for CRISPR Genotyping.- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS).- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR.- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli.- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes.- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus.- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids .- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition.- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl.- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates.- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems.- Cas3 Nuclease-Helicase Activity Assays.- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex.- Creation andAnalysis of a Virome: Using CRISPR Spacers.- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases.- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9.- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

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