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Heterologous Gene Expression in E.coli - Humana Press
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Heterologous Gene Expression in E.coli - hardcover

2010, ISBN: 9781617379666

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvemen… More...

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Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - Evans, Thomas C., Jr. (Editor)
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Evans, Thomas C., Jr. (Editor):

Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - hardcover

2010, ISBN: 1617379662

[EAN: 9781617379666], New book, [PU: Springer Verlag], 310 pages. 10.00x7.00x1.00 inches. In Stock., Books

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Evans, Thomas C., Jr. (Editor):
Heterologous Gene Expression in E.coli: Methods and Protocols: Vol 705 - hardcover

2010

ISBN: 9781617379666

Springer Verlag, 2010. Hardcover. New. 310 pages. 10.00x7.00x1.00 inches., Springer Verlag, 2010, 6

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Jr. Evans:
Heterologous Gene Expression in E.coli : Methods and Protocols - hardcover

2010, ISBN: 1617379662

[EAN: 9781617379666], New book, [PU: Humana], PRINT ON DEMAND Book; New; Fast Shipping from the UK., Books

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Heterologous Gene Expression in E.coli - Jr. Evans, Thomas C.; Ming-Qun Xu
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Jr. Evans, Thomas C.; Ming-Qun Xu:
Heterologous Gene Expression in E.coli - hardcover

2010, ISBN: 9781617379666

Methods and Protocols, Buch, Hardcover, 2011, [PU: Humana Press Inc.], Humana Press Inc., 2010

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Heterologous Gene Expression in E.coli
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9781617379666


Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology(TM) format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.

Details of the book - Heterologous Gene Expression in E.coli


EAN (ISBN-13): 9781617379666
ISBN (ISBN-10): 1617379662
Hardcover
Publishing year: 2011
Publisher: Humana Press Inc.
307 Pages
Weight: 0,758 kg
Language: eng/Englisch

Book in our database since 2007-10-23T17:28:59-04:00 (New York)
Detail page last modified on 2023-07-15T09:04:15-04:00 (New York)
ISBN/EAN: 9781617379666

ISBN - alternate spelling:
1-61737-966-2, 978-1-61737-966-6
Alternate spelling and related search-keywords:
Book author: ming, thomas christopher, thomas evans, qun, chris thomas
Book title: expression, ming, qun

Information from Publisher

Author: Thomas C. Evans, Jr.; Ming-Qun Xu
Title: Methods in Molecular Biology; Heterologous Gene Expression in E.coli - Methods and Protocols
Publisher: Humana; Humana Press
310 Pages
Publishing year: 2010-12-02
Totowa; NJ; US
Printed / Made in
Language: English
160,49 € (DE)
164,99 € (AT)
177,00 CHF (CH)
POD
XI, 310 p.

BB; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; Amino acid residues or moieties; Binding partners; Escherichia coli; Expression hosts; Membrane proteins; Post-translational modifications; Protein expression; Recombinant DNA; Recombinant proteins; Proteomics; Medical Genetics; Protein Biochemistry; Biochemie; EA; BC

Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.
Helps to guide scientists toward an ideal expression host Provides easy to follow protocols, ready to be implemented in the lab Includes tips from the experienced, expert contributors on troubleshooting common methodological pitfalls Includes supplementary material: sn.pub/extras
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