Reverse Chemical Genetics - Paperback

EAN (ISBN-13): 9781617796418
ISBN (ISBN-10): 1617796417
Hardcover
Paperback
Publishing year: 2014
Publisher: Humana
284 Pages
Weight: 0,596 kg
Language: Englisch

Book in our database since 2007-07-19T07:14:31-04:00 (New York)
Detail page last modified on 2023-07-28T23:33:48-04:00 (New York)
ISBN/EAN: 1617796417

ISBN - alternate spelling:
1-61779-641-7, 978-1-61779-641-8
Alternate spelling and related search-keywords:
Book author: hisashi
Book title: molecular genetics

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Information from Publisher

Author: Hisashi Koga
Title: Methods in Molecular Biology; Reverse Chemical Genetics - Methods and Protocols
Publisher: Humana; Humana Press
272 Pages
Publishing year: 2012-05-04
Totowa; NJ; US
Printed / Made in
Language: English
128,39 € (DE)
131,99 € (AT)
142,00 CHF (CH)
POD
XII, 272 p. 64 illus., 4 illus. in color.

BC; Hardcover, Softcover / Medizin/Klinische Fächer; Genetik, Medizin; Verstehen; 2D electrophoresis; Escherichia coli; In situ hybridization; Microarray; PCR; Small molecules; Surface plasmon resonance; Target genes; cDNA libraries; gene expression; genes; genetics; hybridization; molecular biology; Medical Genetics; BB

The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics.- ORFeome Cloning.- Systematic Cloning of an ORFeome Using the Gateway System.- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins.- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study.- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data.- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology.- Modification of cDNA Resources Utilized for Reverse Chemical Genetics.- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources.- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources.- Recombinant Protein Production by a Kaiko–Baculovirus System.- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins.- The Refinement of Established Methods by Recent Innovations.- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine.- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis.- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos.- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins.- Potential Applicable Techniques for Reverse Chemical Genetics.- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases.- Preparation of Highly Sensitive Protein Array Using Reactive Polymer.- High-Throughput SPR Biosensor.- Drug Discovery Through Functional Screening in the Drosophila Heart.- Permeable Cell Assay: A Method for High-ThroughputMeasurement of Cellular ATP Synthetic Activity.- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.
Orchestrates the differential fields of technology in order to create a new era of the study of reverse chemical genetics Introduces the fundamental resources and continues with the most recent molecular technologies Presents applicable techniques and functional assays utilizing specific small molecules